Incipient cytorrhysis
Webincipient cytorrhysis assays indicate that turgor pressure begins to be generated in the appressorium from 16 h (Fig. 1) and penetration of the rice cuticle can occur from 24 h … WebApr 6, 2007 · Appressorial turgor was estimated using incipient cytorrhysis (cell collapse) assays ( 6 ). Conidia in 20-μl droplets (1 × 10 6 conidia/ml) were induced to form …
Incipient cytorrhysis
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WebIn an incipient cytorrhysis (cell collapse) assay using a 1–4 molar concentration of glycerol solution, appressoria of the ΔMomip11 mutant showed increased collapse rates in … WebMar 1, 2024 · The improved incipient-cytorrhysis technology was used to determine the appressorium turgor pressure during different developmental stages (Howard et al. 1991; …
WebMar 1, 2024 · The improved incipient-cytorrhysis technology was used to determine the appressorium turgor pressure during different developmental stages (Howard et al. 1991; Cao et al. 2011). Conidia incubated for different times were treated with different concentrations of PEG-2000 (0.230, 0.225, 0.220, 0.215, 0.210, 0.200, 0.195, and 0.190 … WebJan 15, 2003 · Appressorium turgor can be measured using an incipient cytorrhysis assay which uses hyperosmotic concentrations of a solute to collapse appressoria, thereby allowing estimation of their internal solute concentration and turgor ( Howard et al ., 1991; de Jong et al ., 1997 ).
WebAug 20, 2024 · To elucidate the defect in appressorium penetration caused by myriocin/FB1, we measured the internal turgor pressure within appressoria using an … WebMay 1, 2024 · In the past, several signal transduction pathways regulating different steps of infection-related morphogenesis, including surface recognition, appressorium formation, appressorial penetration, and invasive growth, have been characterized in M. oryzae (Li et al. 2012; Wilson and Talbot 2009).
WebConsistent with the reduction in cuticle penetration, we observed a delay in turgor generation by appressoria when assayed using an incipient cytorrhysis test. This assay measures …
WebIncipient cytorrhysis determined the turgor pressure in appressoria of P. pachyrhizi to be equivalent to 5.13 MPa. MZM data revealed that osmotically active sugar alcohols only … how to remove paint from urethane bumperWebApr 1, 2009 · The appressorium turgor was measured using an incipient cytorrhysis (cell collapse) assay and a 1.0–4.0 M glycerol solution ( Howard et al., 1991 ). Droplets (20 μL) of the conidial suspension (5 × 10 4 spores mL −1) were placed on plastic coverslips and incubated in a humid chamber for 24 h at room temperature. normal corrected calcium levels ukWebFurthermore, we found that the appressorial turgor pressure in the ΔCgscd1 mutant showed no difference compared to that in the WT and Cgscd1com strains in the incipient cytorrhysis experiment. However, fewer infectious hyphae of ΔCgscd1 were observed in the penetration experiments, suggesting that the penetration ability of nonpigmented ... normal corrected calcium levelWebDec 22, 2006 · We therefore estimated the appressorial turgor through the incipient cytorrhysis assays (Howard et al., ... that the Abc3-GFP protein was highly expressed and concentrated in the appressorial plasma membrane right from the incipient stage onwards . However, at a later stage (17 h after germination) during ... normal corrected qtcWebOct 18, 2024 · Panel (A) shows incipient cytorrhysis assays carried out to measure appressorium turgor for the ΔMoOeif3k, ΔMoOeif3k_Com, and wild-type strains inoculated on appressorium-inducing hydrophobic coverslips for 8 h. The appressoria were treated with different concentrations (1, 2, 3, and 4 M) of glycerol solutions. normal cortical sulcation ct headWebDec 27, 2016 · (C) Incipient cytorrhysis assay to measure appressorium turgor generation in Guy11, Δcds1, and Δchk1Δcds1 mutants in the presence or absence of HU exposure … how to remove paint from toilet bowlhow to remove paint from timber